General Lab Duties
- Log needed supplies for order through Quartzy.
- Log received orders on Quartzy, and accurately mark the location and quantity.
- Date all chemicals upon receipt.
Specific Lab Duties
Updated May 9, 2017
|Lab Improvement Managers||Shyam||Identify and see to the completion of lab improvement tasks, of short-, long- and yearly-term scales. Schedule lab improvement meetings to address these. Assign unassigned/irregular/one-time tasks among lab members and hold assignees accountable.|
|Webmaster||Manage lab memberships on all Bennett Lab websites (Slack, Benchling, wiki, Google Group & Drive, Quartzy, Booked) and update the lab directory/roster with new members, permanent email addresses, and reagent initials.|
Maintain the website content: clear junk, upkeep organization and file structure, enforce standards and conventions.
Clear MS Teams clutter and moving worthwhile content to the wiki or Drive.
Maintain Benchling and Drive file structures and conventions. Ensure accurate Quartzy and freezer databases.
Schedule lab meetings, socials, photo sessions, and coordinate with presenters. Update lab calendar with meetings, important seminars, etc.
Ensure lab and lab members comply with safety policy (training and practice). Make safety rules. Liaise with EHS.
Order supplies and receive and store deliveries.
Microscope Room Maintenance
Ensure the scope rooms and their equipment are in working order and clean; communicate with Brian Temple, Kalli Landua, and Eric Rothsgebo.
Maintain cytometer. Run full clean once per week when in use.
|Macroscope Maintenance||Charis||Maintain macroscope and software link. Clean when necessary.|
|Shaker/Incubator Maintenance||All Lab||Clean the shakers when necessary. Manage repairs and upkeep.|
|Centrifuge Maintenance||All Lab||Clean and service centrifuges when necessary. Clean centrifuge bench as necessary. Clean collection tubes? Bleach miniprep waste in fume hood.|
Check gas cylinders. Order new ones when necessary.
|Vacuum Pump Maintenance||Charis|
Communicate tasks to Lucy
Take sealed biohazardous waste boxes to the biohaz waste room in GRB every 1–2 weeks, when ≥3 boxes accrue. Bring back new boxes when supply is low.
Pipette Tip Refills
Refill tip boxes when low.
Autoclave: tip boxes when they are refilled, 8/12/24/96-well plates/blocks (foil-topped), centrifuge bottles,
Gel Area Cleaning
Clean gel area and electrophoresis chambers as necessary, scrubbing chambers with electrodes removed. Make new 50× and 1× TAE when low.
Weighing Bench Cleaning
Clean weigh area, microwave, and stir plate area. Check on supplies (weigh paper/boats, stir bars, spatulas)
Update and backup lab computers, NAS (Meidi); liaise with Rice IT.
Wash all dirty glassware, including culture tubes.
Collect waste beads from benches; clean with 10% bleach. Aliquot into culture tubes and autoclave.
|Agar Plates||Shyam, Xiao, Cole, Charis||Make single-antibiotic plates. Shyam: plain and amp100; Xiao: chl25; Cole: kan50; Charis spt50 (mg/L)|
|Competent Cell Prep||Shyam||Make competent cell aliquots of cloning strains for the lab, NEB Turbo, DH10B.ALT, and DB3.1|
|Common Stock Aliquots||Xiao||10 mM dNTPs, 10× T4 ligase buffer, 1000× antibiotics: chl25, amp100, kan50, spt50 (g/L).|