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1099-1368 PAM-interacting domain (PI)

sgRNA

120 RNA

5'-GGCGCATAAAGATGAGACGCGTTTTAGAGCTATGCTGTTTTGAAAAAAACAGCATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGCTTCG-3'

1-20 guide

10-20 seed

1-41 crRNA

46-118 tracrRNA

DNA

30 dsDNA

5'-GGCGCATAAAGATGAGACGCTGGCGATTAG-3'

3'-CCGCGTATTTCTACTCTGCGACCGCTAATC-5'