...
- 10mM dGTP (can be any other dNTP)
- T4 ligation buffer
- NEBuffer 2.1 (NEBuffer 2 with BSA)1
- At least 18μl of spin column purified dsDNA
BSA2- T4 DNA polymerase
Protocol
...
- Though NEB says that T4 DNA polymerase retains full activity in CutSmart buffer, I believe this references the polymerase activity, not the exonuclease activity. SLIC did not work well with CutSmart buffer, but works very well with Buffer 2.1 -AJH
- NEBuffer 2.1 already contains BSA no additional one is needed