Gel electrophoresis is used to characterize, quantify, and purify DNA by size and topology. Once DNAs have been separated on an agarose gel, desired bands can be excised and purified from the gel by melting the gel fragment and selectively binding its DNA content to a silica column, from which it is eluted. This protocol is based off Qiagen and Zymo gel purification protocols and lab experience.
–Shyam Bhakta
Fragments <4 kb: Incubate at RT 5 min
Fragments 4–10 kb: Incubate at 50°C 5 min
Fragments >10 kb: Incubate at 50°C 10 min