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DNA purification buffers, mostly copied from OpenWetware and Genesee by Shyam Bhakta. <Email for suggestions. Qiagen.com buffers.

Do not autoclave solutions containing ethanol, isopropanol or MOPS; use filter-sterilization if necessary with compatible filter type (nylon for alcohol solns; PES otherwise).
Adjust pH of Tris (base) solutions with HCl.

Buffer P1 — miniprep resuspension buffer, store 4°C after RNase addition, cat#19051
- 50 mM Tris-HCl, pH 8.0
- 10 mM EDTA
- 100 mg/L RNase A
  - 100 mg/mL (1000×) RNase A can be purchased: Qiagen 19101
Buffer P2 — miniprep lysis buffer, cat#19052
- 200 mM NaOH
- 1%_m/V, 10 g/L SDS
- 43 mg/L thymophthalein in ethanol (opt)
Buffer N3 — miniprep neutralization buffer, cat#19064
- 4.2 M Gu-HCl, guanidinium chloride
- 0.9 M KOAc
- HCl → pH 4.8
Buffer PB — DNA-column binding buffer, miniprep opt wash buffer, cat#19066
- 5 M Gu-HCl
- 30%_V/V isopropanol (300 mL/L)
Buffer PE — miniprep final wash buffer, cat#19065
- 10 mM Tris-HCl, pH 7.5
  5× PE base: 50 mM Tris-HCl pH 7.6
  50× PE base: 0.5 M Tris-HCl pH 7.8 (use 20 mL/L + 180 mL/L water)
- 80%_V/V ethanol (800 mL/L)
Buffer EB — miniprep elution buffer
- 10 mM Tris-HCl, pH 8.5
Buffer AE — genomic DNA elution buffer
- 10 mM Tris-HCl, pH 9.0
- 0.5 mM EDTA
Buffer QG — gel solubilization and column binding buffer, cat#19063
- 5.5 M GuSCN, guanidinium thiocyanate
- 20 mM Tris-HCl, pH 6.6
Buffer QX1 — gel solubilization and column binding buffer, cat#20912
- ~~7 M NaPO₄ <insoluble~~
- 7 M NaClO₄
- 10 mM NaOAc, pH 5.3
Buffer P3 — QIAfilter/tip DNA prep neutralization buffer
- 3.0 M KOAc, pH 5.5
  adjust pH with glacial acetic acid, ≈110 mL/L.
Buffer DP3 — Qiagen Directprep 96-well miniprep
- 3.0 M NH₄OAc, pH 5.5
Buffer QXB — for binding >3 kb fragments to columns, cat#19054
- 5 M Gu-HCl
Buffer FWB2 — QIAfilter wash buffer
- 1 M KOAc, pH 5.0
Buffer QBT — QIAfilter equilibration buffer
- 750 mM NaCl
- 50 mM MOPS, pH 7.0
- 15% isopropanol (150 mL/L)
- 0.15%, 1.5 g/L Triton X-100
Buffer QC — QIAfilter/tip wash buffer, cat#19055
- 1.0 M NaCl
- 50 mM MOPS pH 7.0
- 15% isopropanol (150 mL/L)
Buffer QF — QIAfilter elution buffer, cat#19056
- 1.25 M NaCl
- 50 mM Tris-HCl, pH 8.5
- 15% isopropanol (150 mL/L)
Buffer QN — EndoFree QIAfilter elution buffer
- 1.6 M NaCl
- 50 mM MOPS, pH 7.0
- 15% isopropanol (150 mL/L)
Buffer B1 — bacterial lysis buffer
- 50 mM Tris-HCl, pH 8.0
- 50 mM EDTA
- 0.5%, 5 g/L Tween-20
- 0.5%, 5 g/L Triton X-100
- 200 μg/L RNase A
Buffer B2 — bacterial lysis buffer
- 3 M Gu-HCl
- 20%, 200 g/L Tween-20
Buffer C1 — cell lysis buffer, store 4°C
- 1.28 M sucrose
- 40 mM Tris-HCl, pH 7.5
- 20 mM MgCl₂
- 4%, 40 g/L Triton X-100
Buffer G2 — digestion buffer
- 800 mM Gu-HCl
- 30 mM Tris-HCl, pH 8.0
- 30 mM EDTA
- 5%, 50 g/L Tween-20
- 5%, 50 g/L Triton-X100
Buffer Y1 — yeast lysis buffer, store 4̈°C
- 1 M sorbitol
- 100 mM EDTA pH 8.0
- 14 mM β-mercaptoethanol (added just before use)
Buffer PAA — PAGE DNA elution
- 500 mM NH₄OAc
- 100 mM Mg(OAc)₂
- 1 mM EDTA
- 0.1% SDS
Buffer TE — DNA elution/resuspension/storage buffer
- 10 mM Tris-HCl, pH 8.0
- 0.1 or 1 mM EDTA
Buffer STE — DNA resuspension/storage buffer
- 100 mM NaCl
- 10 mM Tris-HCl, pH 8.0
- 1 mM EDTA
LyseBlue®, 1000× — pH indicator, clear→blue ≥pH 9.3
- 43 mg/mL thymophthalein
- in ethanol

Buffer P1 base, 1 L:

1. 6.06 g Tris base
2. 2.922 g EDTA
3. 12 M HCl → pH 8.0, ≈300–400 µL

- Add 50 µL RNase A to each new 50 mL aliquot, stored 4°C.
Buffer P2, 1 L:

1. 8 g NaOH
2. 10 g SDS

Buffer N3, 1 L:

1. 401.2 g Gu-HCl, guanidinium chloride
2. 88.33 g KOAc, potassium acetate
3. 12 M HCl → pH 4.8, ≈32 mL

Buffer PE 50× base (0.5 M Tris-Cl), 0.25 L:

1. 15.14 Tris base
2. 12 M HCl → pH 7.8
3. → 250 mL

Buffer PE, 1 L:

1. 20 mL 50× PE base
2. 180 mL Milli-Q water
3. 800 mL Ethanol (pure), → 1 L