Reviews
CRISPR–Cas9 Structures and Mechanisms
Simulations
Cas9-catalyzed DNA Cleavage Generates Staggered Ends: Evidence from Molecular Dynamics Simulations
Experiments with structural & mechanistic focus.
DNA interrogation by the CRISPR RNA-guided endonuclease Cas9
Biophysical models of specificity.
DNA targeting specificity of RNA-guided Cas9 nucleases (https://crispr.bme.gatech.edu/): Contains experimental data about the specificity. They also have a server based on the data.
A Biophysical Model of CRISPR/Cas9 Activity for Rational Design of Genome Editing and Gene Regulation: This is a simple biophysical model used to predict the specificity of the sgRNA based on the sequence. They split the process in 3 steps and add the free energy change of each process to calculate a binding energy.
High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects : Mutations on residues that bind to the phosphate backbone of the DNA can improve the specificity.
Cleavage
https://www.nature.com/articles/srep37584.pdf
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3110985/
Layman news:
https://www.wired.com/2017/06/crispr-mutations/