General Lab Duties
Date all chemicals upon receipt
- Log needed supplies for order through Quartzy.
- Log received orders on Quartzy, and accurately mark the location and quantity.
- Date all chemicals upon receipt.
Specific Lab Duties
Updated May 9, 2017
TaskTASK | PersonPERSON | Description | ||||
Pipette Tip Refills | Andrew | Refill and autoclave empty tip boxes once per week | ||||
Biohazard Waste | Jennifer | Empty lab biohazard bins every week. Pick up boxes if necessary. | ||||
DESCRIPTION | ||||||
| Lab Improvement Manager | Shyam | Identify and see to the completion of lab improvement tasks. Assign unassigned/irregular/one-time tasks among lab members and hold asignees accountable. | ||||
| Webmaster | Shyam Bhakta | Manage lab members' memberships on all Bennett Lab sites (Slack, Benchling, wiki, Google Group, Quartzy, Booked). Schedule lab meetings and notify presenters. Update lab calendar with meetings, important seminars, etc. Schedule lab photos and socials. | ||||
Safety Officer | Razan -> Shyam | Liaise with EHS. | Lucy | Matt | Communicate tasks to Lucy||
| Ordering | Razan | Order supplies. | ||||
Microscope Room Maintenance | Andrew | /, Razan | /, David | Z.Ensure that the scope rooms are clean | , ; communicate with Brian Temple, Kalli Landua, and Eric Rothsgebo. | |
Cytometer Maintenance | David | Z/, Bárbara | Maintain cytometer. Run full clean once per week when in use. | |||
| Macroscope Maintenance | Bárbara | Maintain macroscope and software link. Clean when necessary. | ||||
| Shaker/Incubator Maintenance | Joanne | Clean the shakers when necessary. Manage repairs and upkeep. | ||||
| Centrifuge Maintenance | Yanfen | Clean and service centrifuges when necessary. Clean centrifuge bench as necessary. Clean collection tubes? Bleach miniprep waste in fume hood. | ||||
Gas Inventory/Ordering | Sara | Check gas cylinders | , order . Order new ones when necessary. | |||
Lucy | Matt | Communicate tasks to Lucy | ||||
Biohazard Waste | Corwin | Take sealed biohazardous waste boxes to the biohaz waste room in GRB every 1–2 weeks, when ≥3 boxes accrue. Bring back new boxes when supply is low. | ||||
Pipette Tip Refills | Andrew | Refill tip boxes when low. | ||||
Autoclaving | David S. | Autoclave washed glassware and tips. Refill and autoclave microfuge tube bins | Meidi | Autoclave: tip boxes when they are refilled, 24/96-well blocks (foil-topped), centrifuge bottles, microcentrifuge tube bins, and occasionally other items on the empty tipo box bench. | ||
Gel Area CleaningGel Area Clean-up | Sara | Clean gel area , dispose of dried gels, put down new bench cover as needed, make new premixed ladder, make new 50X TAEand electrophoresis chambers as necessary, scrubbing chambers with electrodes removed. Make new 50× and 1× TAE when low. | ||||
Weighing Bench CleaningWeigh Area Clean-up | Sara | Clean weigh area, check , microwave, and stir plate area. Check on supplies (weigh paper and /boats, stir bars, spatulas) | ||||
ComputersComputer Upkeep | Andrew/Matt | Update and back-up backup lab computers , and NAS; liaise with IT, NAS. | ||||
Tubes/Glassware Cleaning | Lucy | Wash all dirty glassware | ||||
Safety Officer | Razan | Liase with EHS | ||||
Beads | Barbara | Clean glass beads | ||||
| Centrifuges | Barbara | Clean and service centrifuges when necessary, clean centrifuge bench, clean collection tubes for zymo preps, process miniprep waste bottle in fume hood | ||||
, including culture tubes. | ||||||
Bead Cleaning | Bárbara | Collect waste beads from benches; clean with 10% bleach. Aliquot into culture tubes and autoclave. | ||||
| Webmaster | Shyam | Update lab meeting calendar on wiki and pinned message on Slack, manage membership on all Bennett Lab sites, manage membership on Slack | ||||
| Agar Plates | David Z. / Shyam/ Joanne | Make single-antibiotic plates. (Joanne Amp/Kan, David Z. Chlor/Spec). David: chl34 and spc100. Shyam: amp100. Joanne: kan50. | ||||
| Competent Cell PrepCompetent Cells | Shyam | Make competent cell aliquots of cloning strains for the lab | Shakers | Joanne | Clean the shakers, NEB Turbo, DH10B ALT, and DB3.1 | |
| Aliquots | Jennifer | 10 mM dNTPs, 10× T4 ligase buffer, 1000× antibiotics, | Tubes | Shyam | Wash full baskets of tubes, rack tubes for autoclaving: chl34, amp100, kan50, spc100 (g/L). |