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Minimal Medium

Antibiotic		Stock Concentration		Solvents	Notes	Rich Medium
	Abbr*	Relative	m/V			1× c, Multi-Copy; Range		1× c, Single-Copy	1× c, Single-Copy	1× c, Multi-Copy
Chloramphenicol	chl	1000×	25 mg/mL (formerly	Chloramphenicol	34 mg/mL)	100% Ethanol		25 or 34 mg/L	?		⁽³⁾	≥25–340 mg/L ⁽⁵⁾	10 mg/L ⁽¹⁾
Ampicillin·Na	amp	1000×	100 mg/mL	Water or 40% ethanol or water	40% ethanol EtOH keeps it liquid at -20°C.	100 mg/L? ⁽³⁾		50 mg/L ⁽¹⁾
Kanamycin B·SO₄	kan	1000×	50 mg/mL	35% DMSO or Water	35% DMSO keeps it liquid at -20°C. Insoluble in 40% ethanol or any other solvent.	50 mg/L ⁽³⁾	25 ≥50–500 mg/L ⁽⁵⁾	20 mg/L ⁽¹⁾
Spectinomycin·2HCl·5H₂O	spt	1000×	50 100 mg/mL	Water	to be confirmed: 35% DMSO keeps it liquid at -20°C.	50 mg/L ⁽³⁾	≥50–500 mg/L ⁽⁵⁾	100 20 mg/L ⁽or 50?)?¹⁾
Streptomycin·SO₄	str	1000×	100 mg/mL	Water
Trimethoprim	tmp	1000×	15 mg/mL	DMSO or 1% acetic acid	Insoluble in water or ethanol.	15 mg/L		10 mg/L		⁽²⁾
Apramycin·SO₄	apr	1000×	?	?	?	?		?
Gentamicin·SO₄	gen	1000×	10 mg/mL?	Water or ?		?	?			10 mg/L		10 mg/L ⁽¹⁾
Tetracycline·HCl	tet	1000×Tetracycline	10 mg/mL	Water or ?		10 mg/L ⁽³⁾		?	?		15 mg/L ⁽¹⁾
Hygromycin B·?	hyg	1000×	200 mg/mL	Water		200 mg/L ⁽³⁾

References

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