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Base Strains | ||||||||||
Name | Genotype / Phenotype | Features | Resistances | Auxotrophies | Non-utilizations | |||||
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MG1655 | str. K-12 F– λ– ilvG – rfb-50 rph-1 fnr? | Valine toxicity in absence of isoleucine, due to ilvG frameshift. Partial pyrimidine starvation from polar effects of rph-1 on pyrE causes 9–13% slower growth in minimal medium. Slow growth in M9 galactose (t d=200–300 min) Some strain variants have an fnr deletion that causes defects in anaerobic respiration. P1 phage susceptible due to IS in rfb O-antigen biosythesis gene cluster. genome, allele variations, allele table, MG1655 physiology | — | — | — | |||||
NEB Turbo | str. K-12 F′[ traD36 proA B + lacIq lacZΔM15 fhuA2 ] glnV44(supE44) thi-1 ∆ Δ(lac-proAB) galE15 galK16 R(zgb-210::Tn10)TetS endA1 Δ(hsdS-mcrB)5 (rK –mK –) = str. K-12 TG1[JM101 Δ(hsdS-mcrB )5 (rK– mK–)] endA1] | endonuclease I-deficient (endA1). lacI q. Exceptional competence (galE –). Amber suppressor UAG>CAG(Gln). T1 phage resistant. Blue/ white-screening with lacZα . Non-conjugative F′ (traD36). | Nit R 2dGalR | Thiamine | Galactose, Lactose | |||||
DH10B (Invitrogen) = TOP10 (Thermo) ≈ 10-beta (NEB) | str. K-12 F– Δ(ara-leu)7697[ Δ(rapA'-cra')] Δ(lac)X74[Δ('yahH-mhpE)] duplication(514341-627601)[nmpC-gltI] galK16 galE15 e14 –(icd WT mcrA) φ80dlacZΔM15 recA1 relA1 endA1 Tn10.10 nupG rpsL150(StrR) rph + spoT1 Δ(mrr-hsdRMS-mcrBC)(rK– m K–) λ– NEB 10-beta: [DH10B fhuA] | Recombination-deficient. Relaxed. Endonuclease I-deficient. lacIq. Clones large plasmids. Exceptional competence (galE –). Blue/white-screening with lacZα. Improved cloning 5-mC DNA. P1 phage-resistance. Parent: MC1061 Tandem duplication of genes b0553–b0655; double gene dosage. Genome | StrR 2dGalR | Leucine | Arabinose, Lactose, Galactose | |||||
NEB Stable | str. K-12 DH10B (NEB 10-beta) F'[ proAB lacIq Δ(lacZ)M15 zzf::Tn10 (TetR)] | Shown to exceed performance of Stbl3 in maintenance of repetitive DNAs. Reported genotype is identical to DH10B except with additional tetracycline- and T1 phage-resistances. | Str R TetR 2dGalR | Leucine | Arabinose, Lactose, Galactose | |||||
DH5α | str. K-12 F– endA1 glnV44(supE44) thi-1 recA1 relA1 gyrA96(NalR) nupG purB20 φ80dlacZΔM15 Δ(lacZYA-argF)U169 hsdR17(rK– mK+) , λ– | Recombination-deficient. Relaxed. Endonuclease I-deficient . Clones large plasmids. Amber suppressor UAG>CAG(Gln). Slow growth in M9 from purB-20. Blue/ white-screening with lacZα . | NalR | Thiamine | Lactose | |||||
Mach1 | str. W ΔrecA1398 endA1 fhuA φ80d lacZ Δ M15 Δ(lac)X74 hsdR(rK– mK+) | Recombination-deficient. Endonuclease I-deficient. lacI –. Blue/ white-screening with lacZα . T1/T5/Φ80 phage-resistant. Blue/ white-screening with lacZα . Derivative of str. W (ATCC 9637). Patent: construction/ propertie. Genome. Genome paper. | Lactose | |||||||
BW25113 | str. K-12 F– Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) λ– rph-1 Δ(rhaD-rhaB)568 hsdR514(rK– mK+) | lacI + Keio Keio collection background. Genome. | Arabinose, Rhamnose, Lactose | |||||||
DB3.1 | str. K-12 F– gyrA462(CcdBR) endA1 glnV44 Δ(sr1-recA) mcrB mrr hsdS20(rB–, mB–) araC14 galK2 lacY1 Δ(gpt-proA)62 rpsL20(SmStrR) xylA5 leuB6(Am) mtl-1 lacI–? | CcdB-resistant by gyrase mutation, not CcdA. Recombination-deficient. Endonuclease I-deficient . Amber suppressor UAG>CAG(Gln). | CcdBR StrR 2dGalR | Proline, Leucine | Galactose, Lactose, Xylose, Mannitol, Arabinose? | |||||
OmniMAX2 | str. K-12 F′[proAB+ lacIq lacZΔM15 Tn10(TetR) ΔccdAB] mcrA Δ(mrr-hsdRMS-mcrBC)(rK– mK–) φ80d | lacZΔlacZΔM15 Δ(lacZYA-argF)U169 endA1 | thi-1 | recA1 | relA1 | gyrA96(NalR) fhuA2 panD | CcdB-hypersensitive. T1/T5/Φ80 phage resistant. Recombination-deficient. Relaxed. Endonuclease I-deficient . Clones large plasmids. Amber suppressor UAG>CAG(Gln). Blue/ white-screening with lacZα . | TetR Nal R | Thiamine, Pantothenate | Lactose |
BW29655 | str. K-12 F – Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) λ– Δ (envZ-ompR)520(::FRT) Δ(rhaD-rhaB)568 hsdR514(rK– mK+) rph-1? | lacI + | Arabinose, Lactose, Rhamnose | |||||||
XL1-Blue | str. K-12 | recA1 endA1 gyrA96(NalR) thi- | 1 hsdR17 glnV441 hsdR17 glnV44(supE44) | relA1 lacrelA1 lac F′[proAB+ lacIq | Z∆M15lacZΔM15 Tn10(TetR)] | Recombination-deficient. Relaxed. Endonuclease I-deficient . | lacIq. Amber suppressor UAG>CAG(Gln). Blue/ white-screening with lacZα . | TetR NalR | Thiamine | |
NEB dam – /dcm – | str. K-12 ara-14 leuB6(Am) fhuA31 lacY1 | tsx78tsx78 glnV44(supE44) galK2 galT22 mcrA dcm6 hisG4 rfbD1 R(zgb210::Tn10 | )(TetS) | endA1endA1 rspL136(StrR) dam13::Tn9(ChlR) | xylA5xylA5 mtl-1 thi-1 mcrB1 hsdR2 | Host for isolating plasmids free of Dam/Dcm methylation for transformation into methylation-sensitive hosts. Increased mutation rate as a side-effect. Endonuclease I-deficient . T1/T5/Φ80/T6 phage-resistant. Amber suppressor UAG>CAG(Gln). Low but noticeable frequency of KanR mutations. | ChlR StrR NitR CkaR 2dGalR | Thiamine, Histidine | Galactose, Xylose, Arabinose, Mannitol | |
ET12567 (ATCC BAA-525) | ? | Host for isolating plasmids for transformation into Streptomyces or any other organism that has a methylation-sensitive restriction system. | ? | ? | ? | |||||
EC100D | str. K-12 F– mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 Δ(lac)X74 [Δ('yahH-mhpE)] recA1 endA1 araD139 Δ(ara-leu)7697 galU galK λ– rpsL(StrR) deoR(?) pir+(folA TmpR) | Can replicate conditional R6Kγ origin plasmids using genomic pir. | StrR TmpR 2dGalR | Galactose, Lactose, Arabinose | ||||||
Marionette-Clo | sAJM.1504 [str. DH10B glvC::(Marionette cluster)(ChlR)] | DH10B with Marionette transcription factor cluster. | low ChlR, 5 mg/L | Leucine | Arabinose, Lactose, Galactose | |||||
Marionette-Pro | sAJM.1505 [str. BL21 glvC::(Marionette cluster)(ChlR)] | BL21 with Marionette transcription factor cluster. | low ChlR, 5 mg/L | |||||||
Marionette-Wild | sAJM.1504 [str. K-12 MG1655 glvC::(Marionette cluster)(ChlR)] | MG1655 with Marionette transcription factor cluster. | low ChlR, 5 mg/L | |||||||
BL21(DE3) | str. B F– ompT gal dcm lon hsdSB (rB– mB–) λ(DE3[lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB +]K-12(λS) | lac-inducible T7 RNAP. proteases–. dcm–. | Galactose | |||||||
Rosetta™(DE3)pLysS (Novagen) | str. BL21(DE3) pLysSRARE[T7p20 ileX argU thrU tyrU glyT thrT argW metT leuW proL cat orip15A](ChlR) | lac -inducible T7 RNAP. proteases–. dcm–. pLysSRARE Expresses rare-codon tRNA Arg, Ile, Gly, Leu, Pro : argU(AGG, AGA), ileX(AUA), leuW(CUA), proL(CCC), glyT(GGA), in addition to T7 lysozyme, | from with a p15A ori. T7 lysozyme reduces T7 RNAP background activity and facilitates lysis. | ChlR | Galactose | |||||
BLIM | str. BL26 Blue F– ompT hsdS(rB– mB+) gal dcm lac T1R Parent strain BL26 Blue additionally had F′[proAB lacI q lacZ Δ M15 ::Tn10(TetR)] | Parent strain BL26 Blue (Novagen) was cured of F plasmid and made T1 phage-resistant, according to source. | Proline? | Galactose, Lactose | ||||||
BioDesignER (RE1000) | str. K-12 MG1655 | ilvG+ ΔbioAB::λred[PN25-tetR PtetO2-gam-bet-exo-dam] SS7::cymRq PCP8-araE | PPcon-araC ΔaraBAD | lacIq1 | dnaGQ576A ΔrecJ ΔsbcB | TetR-controlled recombination system. Mutations to stabilize ssDNA. Constitutive lacI, araC, tetR, cymR. Linear ara induction from constitutive araE. Faster minimal media growth from ilvG+. | Biotin | Arabinose | ||
DGF-298 | str. K-12 W3110, Δ(recC ptr recB recD)::Plac-bet | exo kan-exo(KanR) ilvG+ | rph+ | Δ(hella shit) | 35% reduced genome ( 2.98 Mbp from WT 4.6 Mbp). 3.62 Mbp parent strain |
Shyam Can Obtain: | |||||||||||
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Name | Genotype / Phenotype | Features | Resistances | Auxotrophies | Non-utilizations | NEB 10-beta | str. K-12 DH10B fhuA | T1 phage-resistant derivative of DH10B. | StrR 2dGalRLeucine | Arabinose, Lactose, Galactose | |
DH1 | str. K-12 F– λ– endA1 recA1 relA1 gyrA96(NalR) thi-1 glnV44(supE44) hsdR17(rK – mK –) | Recombination-deficient. Relaxed. Endonuclease I-deficient . Efficient 40–60kb plasmid transformer. Amber suppressor UAG>CAG(Gln). DH5α parent | NalR | Thiamine | |||||||
JM109 | str. K-12 endA1 recA1 relA1 gyrA96(NalR) thi-1 glnV44(supE44) Δ(lac-proAB) hsdR17(rK – mK +) F′ [traD36 proAB + lacI q lacZΔM15] | Recombination-deficient. Relaxed. Endonuclease I-deficient . Amber suppressor UAG>CAG(Gln). Blue/ white-screening with lacZα . | NalR | Thiamine | |||||||
BL21(DE3) pLysS | str. BL21(DE3) pLysS[T7p20 ori p15A](ChlR) | lac -inducible T7 RNAP. proteases–. dcm–. pLysS encodes T7 lysozyme, inhibitor of T7 polymerase, which almost eliminates expression from uninduced T7 promoter. | ChlR | Galactose | |||||||
BLR(DE3) (Novagen®) | str. B F– ompT hsdSB (rK – mB –) gal dcm (DE3) Δ(srl-recA)306::Tn10 (TetR) | lac -inducible T7 RNAP. protease OmpT–. Recombination-deficient. dcm–. | TetR | Galactose | |||||||
BLR(DE3) pRIL (Novagen®) | str. B F– ompT hsdSB (rK – mB –) gal dcm λ(DE3 [ lacI lacUV5 - T7p07 ind1 sam7 nin5 ]) Δ(srl-recA)306::Tn10 (TetR) pRIL[argU ileY leuW leuW ori p15A ](Chl R) | lac -inducible T7 RNAP. protease OmpT–. Recombination-deficient. dcm–. pRIL overexpresses rare-codon tRNAArg, Ile, Leu argU (AGA, AGG), ileY (AUA), leuW (CUA) from a p15A replicon. | ChlR TetR | Galactose | |||||||
DP10 | str. DH10B Δ(araFGH) φ(ΔaraEp Pcp8-araE(araE q) | Deletion of Arabinose consumption genes. Constitutive araE. Others, see DH10B. | StrR | Leucine | Arabinose, Lactose | ||||||
RU1012 | str. MH215[MH70[MC4100 [F– [araD139]B/r Δ( lacZYA-argF )U169 e14– flhD5301 Δ(fruK-yeiR)725(fruA25) relA1 rpsL150(StrR) rbsR22 Δ(fimB-fimE)632::IS1 deoC1 λ–] malQ7] Φ(ompC-lacZ)10-25] ΔenvZ::KanR | Relaxed | StrR KanR | Lactose, Fructose | |||||||
JTK165JJ (DIAL strain) | str. MC1061 Δupp:(pir dhfr(TmpR)) Δ(rfbB–wbbL)::repA 1) Δupp::(Pcon(BBa_J23100)-rbs(?)-pir-BBa_B0015(rrfBt-T7_TE) - FRT - Pcon(BBa_J23119)-4krbs-dhfr (TmpR)-tR2 - FRT) 2) Δ(rfbB–wbbL')::(Pcon(BBa_J23119)-rbs(?)-repA-BBa_B0015(rrfBt-T7_TE) - FRT) | relaxed. lacI –. Propagates R6K and ColE2 replicons. Improved cloning 5-mC DNA. | StrR TmpR | Leucine | Galactose, Lactose, Arabinose | ||||||
BW27783 | str. K-12 BW25113 F– λ– Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) Δ(araH-araF)570(::FRT) ΔaraEp-532::FRT φPcp8-araE535 rph-1 Δ(rhaD-rhaB)568 hsdR514 | Constitutive araE (ara transporter) expression, expanding dynamic range of araBp (PBAD) induction. | Arabinose, Rhamnose, Lactose | ||||||||
ET12567 |
BLIM | str. BL26 Blue F– ompT hsdS(rB –mB +) gal dcm lac T1 R base strain BL26 Blue additionally had F′[proAB lacI q lacZΔM15 ::Tn10(TetR)] | BL26 Blue (Novagen) was cured of F plasmid; made T1 phage-resistant, according to source. | Proline? | Galactose, Lactose |