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1. Pipette 8 µl of LB into PCR tubes and 5 ml into glass culture tubes.

2. Add antibiotic to the culture tubes.
3. Pick a colony off the agar plate and mix it into the LB in a PCR tube.
4. Transfer 5 µl of inoculated LB from PCR tube to overnight tube.  Place overnight tubes in 37°C shaker.
5. Make the master mix (volumes given per reaction):
      2.5 µl Taq Buffer
      0.5 µl 10 mM dNTP
      0.5 µl 10 uM F primer
      0.5 µl 10 uM R primer
    17.5 µl water
      0.5 µl Taq polymerase
6. Pipette 22 µl of the master mix into PCR tubes.
7. Place PCR tubes into thermocycler and run the appropriate protocol.
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