Brought to you by and meticulously curated by Shyam. See OpenWetWare for a guide to genotype abbreviations.
Base Strains | |||||
Name | Genotype / Phenotype | Features | Resistances | Auxotrophies | Non-utilizations |
|---|---|---|---|---|---|
| MG1655 | str. K-12 F– λ– ilvG– rfb-50 rph-1 fnr? | Valine toxicity in absence of isoleucine, due to ilvG frameshift. Partial pyrimidine starvation from polar effects of rph-1 on pyrE causes 9–13% slower growth in minimal medium. Slow growth in M9 galactose (td=200–300 min) Some strain variants have an fnr deletion that causes defects in anaerobic respiration. P1 phage susceptible due to IS in rfb O-antigen biosythesis gene cluster. genome, allele variations, allele table, MG1655 physiology | — | — | — |
| NEB Turbo | str. K-12 F′[traD36 proAB+ lacIq lacZΔM15 fhuA2] glnV44(supE44) thi-1 ∆(lac-proAB) galE15 galK16 R(zgb-210::Tn10)TetS endA1 Δ(hsdS-mcrB)5(rK–mK–) = str. K-12 TG1[JM101 Δ(hsdS-mcrB)5(rK–mK–)] endA1] | endonuclease I-deficient (endA1). lacIq. Exceptional competence (galE–). Amber suppressor UAG>CAG(Gln). T1 phage resistant. Blue/white-screening with lacZα. Non-conjugative F′ (traD36). | NitR 2dGalR | Thiamine | Galactose, Lactose |
DH10B (Invitrogen) = TOP10 (Thermo) ≈ 10-beta (NEB) | str. K-12 F– Δ(ara-leu)7697[Δ(rapA'-cra')] Δ(lac)X74[Δ('yahH-mhpE)] duplication(514341-627601)[nmpC-gltI] galK16 galE15 e14–(icdWT mcrA) φ80dlacZΔM15 recA1 relA1 endA1 Tn10.10 nupG rpsL150(StrR) rph+ spoT1 Δ(mrr-hsdRMS-mcrBC)(rK–mK–) λ– NEB 10-beta: [DH10B fhuA] | Recombination-deficient. Relaxed. Endonuclease I-deficient. lacIq. Clones large plasmids. Exceptional competence (galE–). Blue/white-screening with lacZα. Improved cloning 5-mC DNA. P1 phage-resistance. Parent: MC1061 Tandem duplication of genes b0553–b0655; double gene dosage. Genome | StrR 2dGalR | Leucine | Arabinose, Lactose, Galactose |
| NEB Stable | str. K-12 DH10B (NEB 10-beta) F'[proAB lacIq Δ(lacZ)M15 zzf::Tn10(TetR)] | Shown to exceed performance of Stbl3 in maintenance of repetitive DNAs. Reported genotype is identical to DH10B except with additional tetracycline- and T1 phage-resistances. | StrR TetR 2dGalR | Leucine | Arabinose, Lactose, Galactose |
| DH5α | str. K-12 F– endA1 glnV44(supE44) thi-1 recA1 relA1 gyrA96(NalR) nupG purB20 φ80dlacZΔM15 Δ(lacZYA-argF)U169 hsdR17(rK–mK+), λ– | Recombination-deficient. Relaxed. Endonuclease I-deficient. Clones large plasmids. Amber suppressor UAG>CAG(Gln). Slow growth in M9 from purB-20. Blue/white-screening with lacZα. | NalR | Thiamine | Lactose |
| Mach1 | str. W ΔrecA1398 endA1 fhuA φ80dlacZΔM15 Δ(lac)X74 hsdR(rK–mK+) | Recombination-deficient. Endonuclease I-deficient. lacI–. Blue/white-screening with lacZα. T1/T5/Φ80 phage-resistant. Blue/white-screening with lacZα. Derivative of str. W (ATCC 9637). Patent: construction/ propertie. Genome. Genome paper. | Lactose | ||
| BW25113 | str. K-12 F– Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) λ– rph-1 Δ(rhaD-rhaB)568 hsdR514(rK–mK+) | lacI+ Keio collection background. Genome. | Arabinose, Rhamnose, Lactose | ||
| DB3.1 | str. K-12 F– gyrA462(CcdBR) endA1 glnV44 Δ(sr1-recA) mcrB mrr hsdS20(rB–, mB–) araC14 galK2 lacY1 Δ(gpt-proA)62 rpsL20(SmR) xylA5 leuB6(Am) mtl-1 lacI–? | CcdB-resistant by gyrase mutation, not CcdA. Recombination-deficient. Endonuclease I-deficient. Amber suppressor UAG>CAG(Gln). | CcdBR StrR 2dGalR | Proline, Leucine | Galactose, Lactose, Xylose, Mannitol, Arabinose? |
| OmniMAX2 | str. K-12 F′[proAB+ lacIq lacZΔM15 Tn10(TetR) ΔccdAB] mcrA Δ(mrr-hsdRMS-mcrBC)(rK–mK–) φ80dlacZΔM15 Δ(lacZYA-argF)U169 endA1 thi-1 recA1 relA1 gyrA96(NalR) fhuA2 panD | CcdB-hypersensitive. T1/T5/Φ80 phage resistant. Recombination-deficient. Relaxed. Endonuclease I-deficient. Clones large plasmids. Amber suppressor UAG>CAG(Gln). Blue/white-screening with lacZα. | TetR NalR | Thiamine, Pantothenate | Lactose |
| BW29655 | str. K-12 F– Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) λ– Δ(envZ-ompR)520(::FRT) Δ(rhaD-rhaB)568 hsdR514(rK–mK+) rph-1? | lacI+ | Arabinose, Lactose, Rhamnose | ||
| XL1-Blue | str. K-12 recA1 endA1 gyrA96(NalR) thi-1 hsdR17 glnV44(supE44) relA1 lac F′[proAB lacIqZ∆M15 Tn10 TetR] | Recombination-deficient. Relaxed. Endonuclease I-deficient. lacIq. Amber suppressor UAG>CAG(Gln). Blue/white-screening with lacZα. | TetR NalR | Thiamine | |
| NEB dam–/dcm– | ara-14 leuB6(Am) fhuA31 lacY1 tsx78 glnV44(supE44) galK2 galT22 mcrA dcm6 hisG4 rfbD1 R(zgb210::Tn10)(TetS) endA1 rspL136(StrR) dam13::Tn9(ChlR) xylA5 mtl-1 thi-1 mcrB1 hsdR2 | Makes plasmids free of Dam/Dcm methylation; increases mutation rate. Endonuclease I-deficient. T1/T5/Φ80/T6 phage-resistant. Amber suppressor UAG>CAG(Gln). Low but noticeable frequency of KanR mutations. | ChlR StrR NitR CkaR 2dGalR | Thiamine, Histidine | Galactose, Xylose, Arabinose, Mannitol |
| BL21(DE3) | str. B F– ompT gal dcm lon hsdSB(rB–mB–) λ(DE3 [lacI lacUV5-T7p07 ind1sam7 nin5]) [malB+]K-12(λS) | lac-inducible T7 RNAP. proteases–. dcm–. | Galactose | ||
| Rosetta™(DE3)pLysS (Novagen) | BL21(DE3) pLysSRARE[T7p20 ileX argU thrU tyrU glyT thrT argW metT leuW proL cat orip15A](ChlR) | lac-inducible T7 RNAP. proteases–. dcm–. pLysSRARE Expresses rare-codon tRNAArg, Ile, Gly, Leu, Pro: argU(AGG, AGA), ileX(AUA), leuW(CUA), proL(CCC), glyT(GGA), in addition to T7 lysozyme, from p15A ori. T7 lysozyme reduces T7 RNAP background activity and facilitates lysis. | ChlR | Galactose | |
| BioDesignER (RE1000) | str. K-12 MG1655 ilvG+ ΔbioAB::λred[PN25-tetR PtetO2-gam-bet-exo-dam] SS7::cymRq PCP8-araE Pcon-araC ΔaraBAD lacIq1 dnaGQ576A ΔrecJ ΔsbcB | TetR-controlled recombination system. Mutations to stabilize ssDNA. Constitutive lacI, araC, tetR, cymR. Linear ara induction from constitutive araE. Faster minimal media growth from ilvG+. | Biotin | Arabinose | |
| DGF-298 | str. K-12 W3110, Δ(recC ptr recB recD)::Plac-bet exo kan ilvG+ rph+ Δ(hella shit) | 35% reduced genome (2.98 Mbp from WT 4.6 Mbp). 3.62 Mbp parent strain | |||
Shyam Can Obtain: | |||||
|---|---|---|---|---|---|
| Name | Genotype / Phenotype | Features | Resistances | Auxotrophies | Non-utilizations |
| NEB 10-beta | str. K-12 DH10B fhuA | T1 phage-resistant derivative of DH10B. | StrR 2dGalR | Leucine | Arabinose, Lactose, Galactose |
| DH1 | str. K-12 F– λ– endA1 recA1 relA1 gyrA96(NalR) thi-1 glnV44(supE44) hsdR17(rK–mK–) | Recombination-deficient. Relaxed. Endonuclease I-deficient. Efficient 40–60kb plasmid transformer. Amber suppressor UAG>CAG(Gln). DH5α parent | NalR | Thiamine | |
| JM109 | str. K-12 endA1 recA1 relA1 gyrA96(NalR) thi-1 glnV44(supE44) Δ(lac-proAB) hsdR17(rK–mK+) F′ [traD36 proAB+ lacIq lacZΔM15] | Recombination-deficient. Relaxed. Endonuclease I-deficient. Amber suppressor UAG>CAG(Gln). Blue/white-screening with lacZα. | NalR | Thiamine | |
| BL21(DE3) pLysS | str. BL21(DE3) pLysS[T7p20 orip15A](ChlR) | lac-inducible T7 RNAP. proteases–. dcm–. pLysS encodes T7 lysozyme, inhibitor of T7 polymerase, which almost eliminates expression from uninduced T7 promoter. | ChlR | Galactose | |
| BLR(DE3) (Novagen®) | str. B F– ompT hsdSB(rK–mB–) gal dcm (DE3) Δ(srl-recA)306::Tn10 (TetR) | lac-inducible T7 RNAP. protease OmpT–. Recombination-deficient. dcm–. | TetR | Galactose | |
| BLR(DE3) pRIL (Novagen®) | str. B F– ompT hsdSB(rK–mB–) gal dcm λ(DE3 [lacI lacUV5-T7p07 ind1sam7 nin5]) Δ(srl-recA)306::Tn10 (TetR) pRIL[argU ileY leuW orip15A](ChlR) | lac-inducible T7 RNAP. protease OmpT–. Recombination-deficient. dcm–. pRIL overexpresses rare-codon tRNAArg, Ile, Leu argU (AGA, AGG), ileY (AUA), leuW (CUA) from a p15A replicon. | ChlR TetR | Galactose | |
| DP10 | str. DH10B Δ(araFGH) φ(ΔaraEp Pcp8-araE(araEq) | Deletion of Arabinose consumption genes. Constitutive araE. Others, see DH10B. | StrR | Leucine | Arabinose, Lactose |
| RU1012 | str. MH215[MH70[MC4100 [F– [araD139]B/r Δ(lacZYA-argF)U169 e14– flhD5301 Δ(fruK-yeiR)725(fruA25) relA1 rpsL150(StrR) rbsR22 Δ(fimB-fimE)632::IS1deoC1 λ–] malQ7] Φ(ompC-lacZ)10-25] ΔenvZ::KanR | Relaxed | StrR KanR | Lactose, Fructose | |
| JTK165JJ (DIAL strain) | str. MC1061 Δupp:(pir dhfr(TmpR)) Δ(rfbB–wbbL)::repA 1) Δupp::(Pcon(BBa_J23100)-rbs(?)-pir-BBa_B0015(rrfBt-T7_TE) - FRT - Pcon(BBa_J23119)-4krbs-dhfr (TmpR)-tR2 - FRT) 2) Δ(rfbB–wbbL')::(Pcon(BBa_J23119)-rbs(?)-repA-BBa_B0015(rrfBt-T7_TE) - FRT) | relaxed. lacI–. Propagates R6K and ColE2 replicons. Improved cloning 5-mC DNA. | StrR TmpR | Leucine | Galactose, Lactose, Arabinose |
| BW27783 | str. K-12 BW25113 F– λ– Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) Δ(araH-araF)570(::FRT) ΔaraEp-532::FRT φPcp8-araE535 rph-1 Δ(rhaD-rhaB)568hsdR514 | Constitutive araE (ara transporter) expression, expanding dynamic range of araBp (PBAD) induction. | Arabinose, Rhamnose, Lactose | ||