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Antibiotic Stock ConcentrationSolventsNotesRich Medium
 Abbr*Relativem/V  c, Multi-Copy; Rangec, Single-Copy
Chloramphenicolchl1000×

25 mg/mL

(formerly 34 mg/mL)

100% Ethanol 25 or 34 mg/L  (3)≥25–340 mg/L  (5)10 mg/L (1)
Ampicillin·Naamp1000×100 mg/mL40% ethanol or water40% EtOH keeps it liquid at -20°C.100 mg/L  (3) 50 mg/L  (1)
Kanamycin B·SO4 kan1000×50 mg/mL35% DMSO or Water35% DMSO keeps it liquid at -20°C.
Insoluble in 40% ethanol.
50 mg/L  (3)≥50–500 mg/L  (5)20 mg/L  (1)
Spectinomycin·2HCl·5H2O spt1000×50 mg/mLWaterto be confirmed:
35% DMSO keeps it liquid at -20°C.
50 mg/L  (3)≥50–500 mg/L  (5)20 mg/L  (1)
Streptomycin·SO4str1000×100 mg/mLWater    
Trimethoprimtmp1000×15 mg/mLDMSO or 1% acetic acidInsoluble in water or ethanol.15 mg/L 10 mg/L (2)
Apramycin·SO4apr1000×?? ? ?
Gentamicin·SO4gen1000×10 mg/mL?Water or ? 10 mg/L 10 mg/L  (1)
Tetracycline·HCltet1000×10 mg/mLWater or ? 10 mg/L  (3) 15 mg/L  (1)
Hygromycin B·?hyg1000×200 mg/mLWater 200 mg/L  (3)  


References

* Follow American Society of Microbiology abbreviation guidelines when possible

  1. Poteete et al. Biotechniques  (2006)  
  2. Kittleson et al.J Biol Eng (2011)
  3. Addgene
  4. Qiagen
  5. Sara Molinari, unpublished data.
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