Cas9
1380 aa
1-60 RuvCI nuclease
60-94 Arginine rich helix
94-167 RecI
167-307 RecII
307-447 RecI
447-718 RecIII
718-765 RuvCII nuclease
765-909 HNH endonuclease
909-1099 RuvCIII nuclease
1099-1368 PAM-interacting domain (PI)
sgRNA
120 RNA
1-20 guide
10-20 seed
1-41 crRNA
46-118 tracrRNA
DNA
30 dsDNA