1. Pipette 8 µl of LB into PCR tubes and 5 ml into glass culture tubes.
2. Add antibiotic to the culture tubes.
3. Pick a colony off the agar plate and mix it into the LB in a PCR tube.
4. Transfer 5 µl of inoculated LB from PCR tube to overnight tube. Place overnight tubes in 37°C shaker.
5. Make the master mix (volumes given per reaction):
2.5 µl Taq Buffer
0.5 µl 10 mM dNTP
0.5 µl 10 uM F primer
0.5 µl 10 uM R primer
17.5 µl water
0.5 µl Taq polymerase
6. Pipette 22 µl of the master mix into PCR tubes.
7. Place PCR tubes into thermocycler and run the appropriate protocol.