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Brought to you by and meticulously curated by Shyam. See OpenWetWare for a guide to genotype abbreviations.

Base Strains

Name

Genotype / Phenotype

Features

Resistances

Auxotrophies

Non-utilizations

MG1655str. K-12 F– λ– ilvG – rfb-50 rph-1 fnr? Valine toxicity in absence of isoleucine, due to ilvG frameshift.
Partial pyrimidine starvation from polar effects of rph-1 on pyrE causes 9–13% slower growth in minimal medium.
Slow growth in M9 galactose (t d=200–300 min)
Some strain variants have an fnr deletion that causes defects in anaerobic respiration.
P1 phage susceptible due to IS in rfb O-antigen biosythesis gene cluster.
genome, allele variations, allele table, MG1655 physiology		———
NEB Turbostr. K-12 F′[ traD36 proA B + lacIq lacZΔM15 fhuA2 ] glnV44(supE44) thi-1 Δ(lac-proAB) galE15 galK16 R(zgb-210::Tn10)TetS endA1 Δ(hsdS-mcrB)5 (rK –mK –)
= str. K-12 TG1[JM101 Δ(hsdS-mcrB )5 (rK– mK–)] endA1]

endonuclease I-deficient (endA1). lacI q. Exceptional competence (galE –). Amber suppressor UAG>CAG(Gln). T1 phage resistant. Blue/ white-screening with lacZα . Non-conjugative F′ (traD36).
Parent: TG1. Grandparent: JM101.

Nit R 2dGalRThiamineGalactose, Lactose

DH10B (Invitrogen)

= TOP10 (Thermo)

≈ 10-beta (NEB)

str. K-12 F– Δ(ara-leu)7697 Δ(rapA'-cra')] Δ(lac)X74[Δ('yahH-mhpE)] duplication(514341-627601)[nmpC-gltI] galK16 galE15 e14 –(icd WT mcrA) φ80dlacZΔM15 recA1 relA1 endA1 Tn10.10 nupG rpsL150(StrR) rph + spoT1 Δ(mrr-hsdRMS-mcrBC)(rK– m K–) λ–
Missense(dnaA glmS glyQ lpxK mreC murA) Nonsense(chiA gatZ fhuA? yigA ygcG) Frameshift(flhC mglA fruB)

NEB 10-beta: [DH10B fhuA]

Recombination-deficient. Relaxed. Endonuclease I-deficient. lacIq. Clones large plasmids. Exceptional competence (galE –). Blue/white-screening with lacZα. Improved cloning 5-mC DNA. P1 phage-resistance.
Parent: MC1061
Tandem duplication of genes b0553–b0655; double gene dosage.
Genome		StrR 2dGalRLeucineArabinose, Lactose, Galactose
NEB Stablestr. K-12 DH10B (NEB 10-beta) F'[ proAB lacIq Δ(lacZ)M15 zzf::Tn10 (TetR)]Shown to exceed performance of Stbl3 in maintenance of repetitive DNAs. Reported genotype is identical to DH10B except with additional tetracycline- and T1 phage-resistances.Str R TetR 2dGalRLeucineArabinose, Lactose, Galactose
DH5α

str. K-12 F– endA1 glnV44(supE44) thi-1 recA1 relA1 gyrA96(NalR) nupG purB20 φ80dlacZΔM15 Δ(lacZYA-argF)U169 hsdR17(rK– mK+) λ–

Recombination-deficient. Relaxed. Endonuclease I-deficient . Clones large plasmids. Amber suppressor UAG>CAG(Gln). Slow growth in M9 from purB-20. Blue/ white-screening with lacZα .   NalRThiamineLactose
Mach1str. W ΔrecA1398 endA1 fhuA φ80d lacZ Δ M15 Δ(lac)X74 hsdR(rK– mK+)Recombination-deficient. Endonuclease I-deficient. lacI –. Blue/ white-screening with lacZα . T1/T5/Φ80 phage-resistant. Blue/ white-screening with lacZα .
Derivative of str. W (ATCC 9637). Patent: construction/ propertie. Genome. Genome paper.		  Lactose
BW25113str. K-12 F– Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) λ– rph-1 Δ(rhaD-rhaB)568 hsdR514(rK– mK+)lacI +  Keio collection background.
Genome.		  Arabinose, Rhamnose, Lactose
DB3.1str. K-12 F– gyrA462(CcdBR) endA1 glnV44 Δ(sr1-recA) mcrB mrr hsdS20(rB– mB–) araC14 galK2 lacY1 Δ(gpt-proA)62 rpsL20(StrR) xylA5 leuB6(Am) mtl-1 lacI–?CcdB-resistant by gyrase mutation, not CcdA. Recombination-deficient. Endonuclease I-deficient . Amber suppressor UAG>CAG(Gln).CcdBR StrR 2dGalRProline, LeucineGalactose, Lactose, Xylose, Mannitol, Arabinose?
OmniMAX2str. K-12 F′[proAB+ lacIq lacZΔM15 Tn10(TetR) ΔccdAB] mcrA Δ(mrr-hsdRMS-mcrBC)(rK– mK–) φ80dlacZΔM15 Δ(lacZYA-argF)U169 endA1 thi-1 recA1 relA1 gyrA96(NalR) fhuA2 panDCcdB-hypersensitive. T1/T5/Φ80 phage resistant. Recombination-deficient. Relaxed. Endonuclease I-deficient . Clones large plasmids. Amber suppressor UAG>CAG(Gln). Blue/ white-screening with lacZα .TetR Nal RThiamine, PantothenateLactose
BW29655str. K-12 F – Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) λ– Δ (envZ-ompR)520(::FRT) Δ(rhaD-rhaB)568 hsdR514(rK– mK+) rph-1?lacI +  Arabinose, Lactose, Rhamnose
XL1-Bluestr. K-12 recA1 endA1 gyrA96(NalR) thi-1 hsdR17 glnV44(supE44) relA1 lac F′[proAB+ lacIq lacZΔM15 Tn10(TetR)]Recombination-deficient. Relaxed. Endonuclease I-deficient . lacIq. Amber suppressor UAG>CAG(Gln). Blue/ white-screening with lacZα .TetR NalRThiamine 
NEB dam – /dcm – str. K-12 ara-14 leuB6(Am) fhuA31 lacY1 tsx78  glnV44(supE44) galK2 galT22 mcrA dcm6 hisG4 rfbD1 R(zgb210::Tn10(TetS) endA1 rspL136(StrR) dam13::Tn9(ChlR) xylA5 mtl-1 thi-1 mcrB1 hsdR2Host for isolating plasmids free of Dam/Dcm methylation for transformation into methylation-sensitive hosts. Increased mutation rate as a side-effect.
Endonuclease I-deficient . T1/T5/Φ80/T6 phage-resistant. Amber suppressor UAG>CAG(Gln).
Low but noticeable frequency of KanR mutations.		ChlR StrR NitR CkaR 2dGalRThiamine, HistidineGalactose, Xylose, Arabinose, Mannitol
ET12567
(ATCC BAA-525)		?Host for isolating plasmids for transformation into Streptomyces or any other organism that has a methylation-sensitive restriction system.???
EC100Dstr. K-12 F– mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 Δ(lac)X74 [Δ('yahH-mhpE)] recA1 endA1 araD139 Δ(ara-leu)7697 galU galK λ– rpsL(StrR) deoR(?) pir+(folA TmpR)

Can replicate conditional R6Kγ origin plasmids using genomic pir.
Recombination-deficient. Endonuclease I-deficient.

StrR TmpR 2dGalR Galactose, Lactose, Arabinose
Marionette-ClosAJM.1504 [str. DH10B glvC::(Marionette cluster)(ChlR)]DH10B with Marionette transcription factor cluster.low ChlR, 5 mg/LLeucineArabinose, Lactose, Galactose
Marionette-ProsAJM.1505 [str. BL21 glvC::(Marionette cluster)(ChlR)]BL21 with Marionette transcription factor cluster.low ChlR, 5 mg/L  
Marionette-WildsAJM.1504 [str. K-12 MG1655 glvC::(Marionette cluster)(ChlR)]MG1655 with Marionette transcription factor cluster.low ChlR, 5 mg/L  
      
BL21(DE3)str. B F– ompT gal dcm lon hsdSB (rB– mB–) λ(DE3[lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB +]K-12(λS)lac-inducible T7 RNAP. proteases–. dcm–.  Galactose
Rosetta™(DE3)pLysS (Novagen)str. BL21(DE3) pLysSRARE[T7p20 ileX argU thrU tyrU glyT thrT argW metT leuW proL cat orip15A](ChlR)lac -inducible T7 RNAP. proteases–.  dcm–.
pLysSRARE Expresses rare-codon tRNA Arg, Ile, Gly, Leu, Pro : argU(AGG, AGA), ileX(AUA), leuW(CUA), proL(CCC), glyT(GGA), in addition to T7 lysozyme, with a p15A ori.
T7 lysozyme reduces T7 RNAP background activity and facilitates lysis.		ChlR Galactose
BLIMstr. BL26 Blue F– ompT hsdS(rB– mB+) gal dcm lac T1R
Parent strain BL26 Blue additionally had F′[proAB lacI q lacZ Δ M15 ::Tn10(TetR)]		Parent strain BL26 Blue (Novagen) was cured of F plasmid and made T1 phage-resistant, according to source. Proline?Galactose, Lactose
      
BioDesignER (RE1000)str. K-12 MG1655 ilvG+ ΔbioAB::λred[PN25-tetR PtetO2-gam-bet-exo-dam] SS7::cymRq PCP8-araE Pcon-araC ΔaraBAD lacIq1 dnaGQ576A ΔrecJ ΔsbcBTetR-controlled recombination system. Mutations to stabilize ssDNA. Constitutive lacI, araC, tetR, cymR. Linear ara induction from constitutive araE. Faster minimal media growth from ilvG+. BiotinArabinose
DGF-298str. K-12 W3110, Δ(recC ptr recB recD)::Plac-bet-exo(KanR) ilvG+ rph+ Δ(hella shit)35% reduced genome ( 2.98 Mbp from WT 4.6 Mbp). 3.62 Mbp parent strain   

Shyam Can Obtain:

NameGenotype / PhenotypeFeaturesResistancesAuxotrophiesNon-utilizations
DH1str. K-12 F– λ– endA1 recA1 relA1 gyrA96(NalR) thi-1 glnV44(supE44) hsdR17(rK – mK –)Recombination-deficient. Relaxed. Endonuclease I-deficient . Efficient 40–60kb plasmid transformer. Amber suppressor UAG>CAG(Gln).
DH5α parent		NalRThiamine 
JM109str. K-12 endA1 recA1 relA1 gyrA96(NalR) thi-1 glnV44(supE44) Δ(lac-proAB) hsdR17(rK – mK +) F′ [traD36 proAB + lacI q lacZΔM15]Recombination-deficient. Relaxed. Endonuclease I-deficient . Amber suppressor UAG>CAG(Gln). Blue/ white-screening with lacZα .NalRThiamine 
BL21(DE3) pLysSstr. BL21(DE3) pLysS[T7p20 ori p15A](ChlR)lac -inducible T7 RNAP. proteases–.  dcm–.
pLysS encodes T7 lysozyme, inhibitor of T7 polymerase, which almost eliminates expression from uninduced T7 promoter.		ChlR Galactose
BLR(DE3) (Novagen®)str. B F– ompT hsdSB (rK – mB –) gal dcm (DE3) Δ(srl-recA)306::Tn10 (TetR)lac -inducible T7 RNAP. protease OmpT–. Recombination-deficient.  dcm–.TetR Galactose
BLR(DE3) pRIL (Novagen®)str. B F– ompT hsdSB (rK – mB –) gal dcm λ(DE3 [ lacI   lacUV5 - T7p07   ind1 sam7   nin5 ]) Δ(srl-recA)306::Tn10 (TetR) pRIL[argU ileY leuW  ori p15A ](Chl R)lac -inducible T7 RNAP. protease OmpT–. Recombination-deficient.  dcm–.
pRIL overexpresses rare-codon tRNAArg, Ile, Leu argU (AGA, AGG), ileY (AUA), leuW (CUA) from a p15A replicon.		ChlR TetR Galactose
DP10str. DH10B Δ(araFGH) φ(ΔaraEp Pcp8-araE(araE q)Deletion of Arabinose consumption genes. Constitutive araE. Others, see DH10B.StrRLeucineArabinose, Lactose
RU1012str. MH215[MH70[MC4100 [F– [araD139]B/r Δ( lacZYA-argF )U169 e14– flhD5301 Δ(fruK-yeiR)725(fruA25) relA1 rpsL150(StrR) rbsR22 Δ(fimB-fimE)632::IS1 deoC1 λ–] malQ7] Φ(ompC-lacZ)10-25] ΔenvZ::KanRRelaxedStrR KanR Lactose, Fructose
JTK165JJ (DIAL strain)str. MC1061 Δupp:(pir dhfr(TmpR)) Δ(rfbB–wbbL)::repA

1) Δupp::(Pcon(BBa_J23100)-rbs(?)-pir-BBa_B0015(rrfBt-T7_TE) - FRT - Pcon(BBa_J23119)-4krbs-dhfr (TmpR)-tR2 - FRT)
2) Δ(rfbB–wbbL')::(Pcon(BBa_J23119)-rbs(?)-repA-BBa_B0015(rrfBt-T7_TE) - FRT)		relaxed. lacI –. Propagates R6K and ColE2 replicons. Improved cloning 5-mC DNA.StrR TmpRLeucineGalactose, Lactose, Arabinose
BW27783str. K-12 BW25113 F– λ– Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) Δ(araH-araF)570(::FRT) ΔaraEp-532::FRT φPcp8-araE535 rph-1 Δ(rhaD-rhaB)568 hsdR514Constitutive araE (ara transporter) expression, expanding dynamic range of araBp (PBAD) induction.  Arabinose, Rhamnose, Lactose
      

 

 

ET12567

 

 

BLIMstr. BL26 Blue F– ompT hsdS(rB –mB +) gal dcm lac T1 R
base strain BL26 Blue additionally had F′[proAB lacI q lacZΔM15 ::Tn10(TetR)]		BL26 Blue (Novagen) was cured of F plasmid; made T1 phage-resistant, according to source. Proline?Galactose, Lactose
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