Original Hg light source was replaced with LED light source. Equivalent powers were determined using Chroma fluorescent slides at 60x using the test exposure times.
Fluorescence Channel | Test Exposure Time | Average Camera Readout at 10% of Hg Lamp | Percent Power of LED Source |
---|---|---|---|
CFP | 300 ms | ~1,430 | 8% |
GFP | 1 ms | ~4,000 | 4% |
YFP | 10 ms | ~4,130 | 12% |
mCherry | 1 ms | ~10,900-11,000 | 19% |