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Stock, conc	Preparation & Storage	Volumes, Tubes	StorageLocation
10× T4 ligase buffer	Thawed, precipitates resuspended with vortexing and/or brief 37° incubation.	50 µL aliquots	-20°, rack Rack 1.5
10 mM dNTPs	?Thawed	20 µL aliquots	-20°, rack Rack 1.5
Antibiotics
chl^{25 (mg/mL)}	Ethanol, -20°; stable for ≥1 year.	15 mL	-20°, freezer Freezer 1 door
amp¹⁰⁰	40% ethanol, -20°; seems to be stable for ≥6 mo for agar	15 mL	-20°, freezer Freezer 1 door
kan⁵⁰	Water, 0°; stable for ≥1 year.	15 mL	-20°, freezer Freezer 0 door
spt⁵⁰	Water, 0°; stable for ≥1 year.	15 mL	Freezer 0 door
gen	Water, -20°, freezer 0 door; stable for…	1 mL	Rack 1.5

Antibiotics

Antibiotic	Target		Stock Concentration		Solvents	Notes	Rich Medium
		Abbr*	Relative	m/V			1× c, Multi-Copy; Range		1× c, Single-Copy
Chloramphenicol	^ribosome	chl	1000×	25 mg/mL (formerly 34 mg/mL)	100% Ethanol		25 or 34 mg/L ⁽³⁾	≥25–340 mg/L ⁽⁵⁾	10 mg/L ⁽¹⁾ or 5 mg/L ⁽⁶⁾
Ampicillin·Na	^{peptidoglycan}	amp car	1000×	100 mg/mL	40% ethanol or water	^{40% EtOH keeps it liquid at -20°C.} ^{Substitutes carbenicillin.}	100 mg/L ⁽³⁾	≥100–200 mg/L	50 mg/L ⁽¹⁾
Kanamycin B·SO₄	^ribosome	kan	1000×	50 mg/mL	35% DMSO or Water	^{Insoluble in 40% ethanol.} ^{Precipitates from 35% DMSO.}	50 mg/L ⁽³⁾	≥50–500 mg/L ⁽⁵⁾	20 mg/L ⁽¹⁾
Spectinomycin·2HCl·5H₂O	^ribosome	spt	1000×	50 mg/mL	Water	^{Precipitates from 35% DMSO.}	50 mg/L ⁽³⁾	≥50–500 mg/L ⁽⁵⁾	20 mg/L ⁽¹⁾
Streptomycin·SO ₄	^ribosome	str	1000×	100 mg/mL	Water
Trimethoprim	^{FolA (DHFR)}	tmp	1000×	15 mg/mL	DMSO or 1% acetic acid	^{Insoluble in water or ethanol. Exogenous thymine can lead to evolution of resistance.}	15 mg/L?		10 mg/L ⁽²⁾
Apramycin·SO ₄	^ribosome	apr	1000×	?	Water, presumably		?		?
Gentamicin·SO ₄	^ribosome	gen	1000×	10 mg/mL?	Water or ?		10 mg/L⁽⁸⁾		10 mg/L ⁽¹⁾
Erythromycin	^ribosome	ery
Tetracycline·HCl	^ribosome	tet	1000×	10 mg/mL	Water or ?	^{Light-sensitive.}	10 mg/L ⁽³⁾		15 mg/L ⁽¹⁾
Zeocin (Phleomycin D1 · Cu²⁺)	^DNA	zeo	1000×	25 mg/mL	Water	^{Use with LB-Lennox (not >5 g/L NaCl), pH 7.5. Light-sensitive.}	25 mg/L⁽⁷⁾	25–50 mg/L ⁽⁷⁾
Hygromycin BHygromycin B?	^ribosome	hyg	1000×	200 mg/mL	Water		200 mg/L ⁽³⁾

References

* Follow American Society of Microbiology AAC abbreviation guidelines when possible

Poteete et al., Biotechniques (2006)
Kittleson et al., J Biol Eng (2011)
Addgene
Qiagen
Sara Molinari, unpublished data, p15A with cat, aphA1, aadA.
Addgene: Marionette Sensor Collection
Zeocin | Thermo Fisher Scientific

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Antibiotics