Common Stocks

Stock, conc	Preparation	Volumes, Tubes	Storage
10× T4 ligase buffer	Thawed, precipitates resuspended with vortexing and/or brief 37° incubation.	50 µL aliquots	-20°, rack 1.5
10 mM dNTPs	?	20 µL aliquots	-20°, rack 1.5
Antibiotics
chl^{25 (mg/mL)}	Ethanol, -20°; stable for ≥1 year.	15 mL	-20°, freezer 1 door
amp¹⁰⁰	40% ethanol, -20°; seems to be stable for ≥6 mo for agar	15 mL	-20°, freezer 1 door
kan⁵⁰	Water, 0°; stable for ≥1 year.	15 mL	-20°, freezer 0 door
spt⁵⁰	Water, 0°; stable for ≥1 year.	15 mL	-20°, freezer 0 door

Antibiotics

Antibiotic	Target		Stock Concentration		Solvents	Notes	Rich Medium
		Abbr*	Relative	m/V			1× c, Multi-Copy; Range		1× c, Single-Copy
Chloramphenicol	^ribosome	chl	1000×	25 mg/mL (formerly 34 mg/mL)	100% Ethanol		25 or 34 mg/L ⁽³⁾	≥25–340 mg/L ⁽⁵⁾	10 mg/L ⁽¹⁾ or 5 mg/L ⁽⁶⁾
Ampicillin·Na	^{peptidoglycan}	amp	1000×	100 mg/mL	40% ethanol or water	40% EtOH keeps it liquid at -20°C. Substitutes carbenicillin.	100 mg/L ⁽³⁾		50 mg/L ⁽¹⁾
Kanamycin B·SO₄	^ribosome	kan	1000×	50 mg/mL	35% DMSO or Water	Insoluble in 40% ethanol. Precipitates from 35% DMSO.	50 mg/L ⁽³⁾	≥50–500 mg/L ⁽⁵⁾	20 mg/L ⁽¹⁾
Spectinomycin·2HCl·5H₂O	^ribosome	spt	1000×	50 mg/mL	Water	Precipitates from 35% DMSO.	50 mg/L ⁽³⁾	≥50–500 mg/L ⁽⁵⁾	20 mg/L ⁽¹⁾
Streptomycin·SO₄	^ribosome	str	1000×	100 mg/mL	Water
Trimethoprim	^{FolA (DHFR)}	tmp	1000×	15 mg/mL	DMSO or 1% acetic acid	Insoluble in water or ethanol.	15 mg/L?		10 mg/L ⁽²⁾
Apramycin·SO₄	^ribosome	apr	1000×	?	Water, presumably		?		?
Gentamicin·SO₄	^ribosome	gen	1000×	10 mg/mL?	Water or ?		10 mg/L		10 mg/L ⁽¹⁾
Tetracycline·HCl	^ribosome	tet	1000×	10 mg/mL	Water or ?		10 mg/L ⁽³⁾		15 mg/L ⁽¹⁾
Hygromycin B?	^ribosome	hyg	1000×	200 mg/mL	Water		200 mg/L ⁽³⁾

References