PCR
DNA Agarose Gel Electrophoresis
DNA Purification
DNA Assembly
DNA Transformation
Heat/chemical shock transformation (TSS method, common stocks)
Heat/chemical transformation (Inoue method)
Old heat/chemical shock transformation (Knight Lab TSS method)
Old Old 5× KCM + TSS Transformation Protocol
Sucrose-wash electrotransformation
E. coli genome integration with λ red
Genotyping