Brought to you by and meticulously curated by Shyam. See OpenWetWare for a guide to genotype abbreviations.
Base Strains |
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Name | Genotype / Phenotype | Features | Resistances | Auxotrophies | Non-utilizations | ||||||||
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MG1655 | str. K-12 F– λ– ilvG– rfb-50 rph-1 fnr? | Valine toxicity in absence of isoleucine, due to ilvG frameshift. Partial pyrimidine starvation from polar effects of rph-1 on pyrE causes 9–13% slower growth in minimal medium. Slow growth in M9 galactose (td=200–300 min) Some strain variants have an fnr deletion that causes defects in anaerobic respiration. P1 phage susceptible due to IS in rfb O-antigen biosythesis gene cluster. genome, allele variations, allele table, MG1655 physiology | — | — | — | ||||||||
NEB Turbo | str. K-12 F′[traD36 proAB+ lacIq lacZΔM15 fhuA2] glnV44(supE44) thi-1 ∆(lac-proAB) galE15 galK16 R(zgb-210::Tn10)TetS endA1 Δ(hsdS-mcrB)5, (rK–mK–) F′[proA+B+ lacIqlacZΔM15/fhuA2] = str. K-12 TG1[JM101 Δ(hsdS-mcrB)5(rK–mK–)] endA1] | endonuclease I-deficient (endA1) endonuclease–. lacIq. Exceptional competence (galE–). Amber suppressor UAG>CAG(Gln). T1 phage resistant. Blue/white-screening with lacZα. Non-conjugative F′ (traD36). | NitR 2dGalR | Thiamine | Galactose, Lactose | ||||||||
DH10B (Invitrogen) = TOP10 (Thermo) ≈ 10-beta (NEB) | str. K-12 F– Δ(ara-leu)7697 araD139 ΔlacX74 [Δ(rapA'-cra')] Δ(lac)X74[Δ('yahH-mhpE)] duplication(514341-627601)[nmpC-gltI] galK16 galE15 deoR+ e14–(icdWT mcrA) mcrA galU+ φ80dlacZΔM15 recA1 relA1 endA1 Tn10.10 nupG rpsL150(StrR) rph+ spoT1 Δ(mrr-hsdRMS-mcrBC)(rK–mK–) λ– NEB 10-beta: [DH10B fhuA] | Recombination-deficient. Relaxed. Endonuclease –I-deficient. lacIq. Clones large plasmids. Exceptional competence (galE–). P1 phage-resistanceBlue/white-screening with lacZα. Improved cloning 5-mC DNA. P1 phage-resistance. Parent: MC1061 Tandem duplication of genes b0553–b0655; double gene dosage. Genome | StrR 2dGalR | Leucine | Arabinose, Lactose, (?)Galactose | ||||||||
NEB Stable | str. K-12 DH10B (NEB 10-beta) F'[proAB lacIq Δ(lacZ)M15 zzf::Tn10(TetR)] | Shown to exceed performance of Stbl3 in maintenance of repetitive DNAs. Reported genotype is identical to DH10B except with additional tetracycline- and T1 phage-resistances. | StrR TetR 2dGalR | Leucine | Arabinose, Lactose, Galactose | ||||||||
DH5α | str. K-12 F– endA1 glnV44(supE44) thi-1 recA1 relA1 gyrA96(NalR) deoR+ nupG purB20 φ80dlacZΔM15 Δ(lacZYA-argF)U169 , hsdR17(rK–mK+), λ– | Recombination-deficient. Relaxed. Endonuclease –I-deficient. Clones large plasmids. Amber suppressor UAG>CAG(Gln). Slow growth in M9 from purB-20. Blue/white-screening with lacZα. | NalR | Thiamine, Arginine | Lactose | ||||||||
Mach1 | str. W ΔrecA1398 endA1 fhuA Φ80Δ(lac) φ80dlacZΔM15 Δ(lac)X74 hsdR(rK–mK+) | Recombination-deficient. Endonuclease I-deficient. lacI–. lacZα-complementer Blue/white-screening with lacZα. T1/T5/Φ80 phage-resistant. Blue/white-screening with lacZα. Derivative of str. W (ATCC 9637). Patent: construction/ propertie. Genome. Genome paper. | Lactose | ||||||||||
BW25113 | str. K-12 F– Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) λ– rph-1 Δ(rhaD-rhaB)568 hsdR514(rK–mK+) | lacI+ Keio collection background. Genome. | Arabinose, Rhamnose, Lactose | ||||||||||
DB3.1 | str. K-12 F– gyrA462(CcdBR) endA1 glnV44 Δ(sr1-recA) mcrB mrr hsdS20(rB–, mB–) ara14 araC14 galK2 lacY1 proA2 Δ(gpt-proA)62 rpsL20(SmR) xyl5 leu( xylA5 leuB6(Am) ?) mtl1 mtl-1 lacI–? | CcdB-resistant by gyrase mutation, not CcdA. Recombination-deficient. Endonuclease – I-deficient. Amber suppressor UAG>CAG(Gln). | CcdBR StrR 2dGalR | Proline, Leucine | Galactose, Lactose, Arabinose, Xylose, Mannitol, Arabinose? | ||||||||
OmniMAX2 | str. K-12 F′[proAB+ lacIq lacZΔM15 Tn10(TetR) ΔccdAB] mcrA Δ(mrr-hsdRMS-mcrBC)(rK–mK–) φ80dlacZΔM15 Δ(lacZYA-argF)U169 endA1 thi-1 recA1 relA1 gyrA96(NalR) fhuA2 panD | CcdB-hypersensitive. T1/T5/Φ80 phage resistant. Recombination-deficient. Relaxed. Endonuclease I-deficient. Clones large plasmids. Amber suppressor UAG>CAG(Gln). Blue/white-screening with lacZα. | TetR NalR | Thiamine, Pantothenate | Lactose | ||||||||
BW29655 | str. K-12 F– Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) λ– Δ(envZ-ompR)520(::FRT) Δ(rhaD-rhaB)568 hsdR514(rK–mK+) rph-1? | lacI+ | Arabinose, Lactose, Rhamnose | ||||||||||
Shyam Can Obtain: | |||||||||||||
Name | Genotype / Phenotype | Features | Resistances | Auxotrophies | Non-utilizations | ||||||||
XL1-Blue | str. K-12 | F– λ– endA1 recA1 relA1 gyrA96recA1 endA1 gyrA96(NalR) | thithi- | 11 hsdR17 glnV44(supE44) | hsdR17(rK–mK–)relA1 lac F′[proAB lacIqZ∆M15 Tn10 TetR] | Recombination-deficient. Relaxed. Endonuclease | –. Efficient 40–60kb plasmid transformerI-deficient. lacIq. Amber suppressor UAG>CAG(Gln) | . Blue/white-screening with lacZα. | TetR NalR | Thiamine | JM109 | str. K-12 endA1 recA1 relA1 gyrA96(NalR) thi-1 glnV44(supE44) Δ(lac-proAB) hsdR17(rK–mK+) F′ [traD36 proAB+ lacIq lacZΔM15] | Recombination-deficient. Relaxed. Endonuclease–|
NEB dam–/dcm– | ara-14 leuB6(Am) fhuA31 lacY1 tsx78 glnV44(supE44) galK2 galT22 mcrA dcm6 hisG4 rfbD1 R(zgb210::Tn10)(TetS) endA1 rspL136(StrR) dam13::Tn9(ChlR) xylA5 mtl-1 thi-1 mcrB1 hsdR2 | Makes plasmids free of Dam/Dcm methylation; increases mutation rate. Endonuclease I-deficient. T1/T5/Φ80/T6 phage-resistant. Amber suppressor UAG>CAG(Gln) | Nal. Low but noticeable frequency of KanR mutations. | ChlR StrR NitR CkaR 2dGalR | Thiamine | , Histidine | Galactose, Xylose, Arabinose, Mannitol | ||||||
BL21(DE3) | str. B F– ompT gal dcm lon hsdSB(rB–mB–) λ(DE3 [lacI lacUV5-T7p07 ind1sam7 nin5]) [malB+]K-12(λS) | proteases–;lac-inducible T7 RNAP | Galactose | BL21(DE3) pLysS | str. | BL21(DE3) pLysS[T7p20 orip15A](ChlR)proteases–; lac-inducible T7 RNAP pLysS encodes T7 lysozyme, inhibitor of T7 polymerase, which almost eliminates expression from uninduced T7 promoter. | proteases–. dcm–. | ChlR | Galactose | ||||
Rosetta™(DE3)pLysS (Novagen) | BL21(DE3) pLysSRARE[T7p20 ileX argU thrU tyrU glyT thrT argW metT leuW proL cat orip15A](ChlR) | proteases–;lac-inducible T7 RNAP. proteases–. dcm–. pLysSRARE Expresses rare-codon tRNAArg, Ile, Gly, Leu | and , Pro | -tRNAs,: argU(AGG, AGA), ileX(AUA), leuW(CUA), proL(CCC), glyT(GGA), in addition to T7 lysozyme, from p15A ori. T7 lysozyme reduces T7 RNAP background activity and facilitates lysis. | ChlR | Galactose | |||||||
BioDesignER (RE1000) | str. K-12 MG1655 ilvG+ ΔbioAB::λred[PN25-tetR PtetO2-gam-bet-exo-dam] SS7::cymRq PCP8-araE Pcon-araC ΔaraBAD lacIq1 dnaGQ576A ΔrecJ ΔsbcB | TetR-controlled recombination system. Mutations to stabilize ssDNA. Constitutive lacI, araC, tetR, cymR. Linear ara induction from constitutive araE. Faster minimal media growth from ilvG+. | Biotin | Arabinose | |||||||||
DGF-298 | str. K-12 W3110, Δ(recC ptr recB recD)::Plac-bet exo kan ilvG+ rph+ Δ(hella shit) | 35% reduced genome (2.98 Mbp from WT 4.6 Mbp). 3.62 Mbp parent strain |
Shyam Can Obtain: | |||||||||||
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Name | Genotype / Phenotype | Features | Resistances | Auxotrophies | Non-utilizations | ||||||
NEB 10-beta | str. K-12 DH10B fhuA | T1 phage-resistant derivative of DH10B. | StrR 2dGalR | Leucine | Arabinose, Lactose, Galactose | ||||||
DH1 | str. K-12 F– λ– endA1 recA1 relA1 gyrA96(NalR) thi-1 glnV44(supE44) hsdR17(rK–mK–) | Recombination-deficient. Relaxed. Endonuclease I-deficient. Efficient 40–60kb plasmid transformer. Amber suppressor UAG>CAG(Gln). DH5α parent | NalR | Thiamine | |||||||
JM109 | str. K-12 endA1 recA1 relA1 gyrA96(NalR) thi-1 glnV44(supE44) Δ(lac-proAB) hsdR17(rK–mK+) F′ [traD36 proAB+ lacIq lacZΔM15] | Recombination-deficient. Relaxed. Endonuclease I-deficient. Amber suppressor UAG>CAG(Gln). Blue/white-screening with lacZα. | NalR | Thiamine | |||||||
BL21(DE3) pLysS | str. BL21(DE3) pLysS[T7p20 orip15A](ChlR) | lac-inducible T7 RNAP. proteases–. dcm–. pLysS encodes T7 lysozyme, inhibitor of T7 polymerase, which almost eliminates expression from uninduced T7 promoter. | ChlR | Galactose | |||||||
BLR(DE3) (Novagen®) | str. B F– ompT hsdSB(rK–mB–) gal dcm (DE3) Δ(srl-recA)306::Tn10 (TetR) | lac-inducible T7 RNAP. protease OmpT–. Recombination-deficient. dcm–. | TetR | Galactose | |||||||
BLR(DE3) pRIL (Novagen®) | str. B F– ompT hsdSB(rK–mB–) gal dcm λ(DE3 [lacI lacUV5-T7p07 ind1sam7 nin5]) Δ(srl-recA)306::Tn10 (TetR) pRIL[argU ileY leuW orip15A](ChlR) | lac-inducible T7 RNAP. protease OmpT–. Recombination-deficient. dcm–. Overexpresses pRIL overexpresses rare-codon tRNAArg, Ile, Leu argU (AGA, AGG), ileY (AUA), leuW (CUA) from a p15A replicon. | ChlR TetR | Galactose | |||||||
DP10 | str. DH10B Δ(araFGH) φ(ΔaraEp Pcp8-araE(araEq) | Deletion of Arabinose consumption genes. Constitutive araE. Others, see DH10B. | StrR | Leucine | Arabinose, Lactose | ||||||
RU1012 | str. MH215[MH70[MC4100 [F– [araD139]B/r Δ(lacZYA-argF-lac)169U169 e14– flhD5301 Δ(fruK-yeiR)725(fruA25) relA1 rpsL150(StrR) rbsR22 Δ(fimB-fimE)632::IS1deoC1 λ–] malQ7] Φ(ompC-lacZ)10-25] ΔenvZ::KanR | Relaxed | StrR KanR | Arginine | Lactose, Fructose | ||||||
JTK165JJ (DIAL strain) | str. MC1061 Δupp:(pir dhfr(TmpR)) Δ(rfbB–wbbL)::repA 1) Δupp::(Pcon(BBa_J23100)-rbs(?)-pir-BBa_B0015(rrfBt-T7_TE) - FRT - Pcon(BBa_J23119)-4krbs-dhfr (TmpR)-tR2 - FRT) 2) Δ(rfbB–wbbL')::(Pcon(BBa_J23119)-rbs(?)-repA-BBa_B0015(rrfBt-T7_TE) - FRT) | relaxed. lacI–. Propagates R6K and ColE2 replicons. Improved cloning 5-mC DNA. | StrR TmpR | Leucine | Galactose, Lactose, Arabinose | DGF-298 3.62 Mbp source strain | str. K-12 W3110, Δ(recC ptr recB recD)::Plac-bet exo kan ilvG+ rph+ Δ(hella shit) | 2.98 Mbp reduced genome (from 4.6 Mbp) | - | - | - |
BW27783 | str. K-12 BW25113 F– λ– Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) Δ(araH-araF)570(::FRT) ΔaraEp-532::FRT φPcp8-araE535 rph-1 Δ(rhaD-rhaB)568hsdR514 | Constitutive araE (ara transporter) expression, expanding dynamic range of araBp (PBAD) induction. | - - | Arabinose, Rhamnose, Lactose | |||||||
RE630 | EcNR1[MG1655 bla bio λ-Red1] ilvG+ dam@λTerm tetO2-pTet<>{kil, λ cI}cymR<>SS7 araEq araCq lacIq-1 recJ sbcB dnaG-Q576A | aTc-inducible recombination machinery. Recombination-deficient. Mutations to stabilize ssDNA | AmpR | Biotin | |||||||