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NameGenotype / PhenotypeFeaturesResistancesAuxotrophiesNon-utilizations
MG1655str. K-12 F– λ– ilvG– rfb-50 rph-1 fnr?Valine toxicity in absence of isoleucine, due to ilvG frameshift.
Partial pyrimidine starvation from polar effects of rph-1 on pyrE causes 9–13% slower growth in minimal medium.
Slow growth in M9 galactose (td=200–300 min)
Some strain variants have an fnr deletion that causes defects in anaerobic respiration.
P1 phage susceptible due to IS in rfb O-antigen biosythesis gene cluster.
genome allele variations MG1655 physiology		   
NEB Turbostr. K-12 glnV44(supE44) thi-1 ∆(lac-proAB) galE15 galK16 R(zgb-210::Tn10)TetS endA1 Δ(hsdS-mcrB)5, (rK–mK–) F′[proA+B+ lacIqlacZΔM15/fhuA2]endonuclease–. lacIq. Exceptional competence (galE–). Amber suppressor UAG>CAG(Gln). T1 phage resistantNitR 2dGalRThiamineGalactose, Lactose
DH10B (Invitrogen)

str. K-12 F– Δ(ara leu)7697 araD139 ΔlacX74 duplication(514341-627601) galK16 galE15 deoR+ e14–(icdWT) mcrA galU+ φ80dlacZΔM15 recA1 relA1endA1 Tn10.10 nupG rpsL150(StrR) rph+ spoT1 Δ(mrr-hsdRMS-mcrBC)(rK–mK–) λ–
Missense(dnaA glmS glyQ lpxK mreC murA)
Nonsense(chiA gatZ fhuA? yigA ygcG)
Frameshift(flhC mglA fruB)

Recombination-deficient. Relaxed. Endonuclease–. lacIq. Clones large plasmids. Exceptional competence (galE–). P1 phage-resistance. Improved cloning 5-mC DNA.
Tandem duplication of genes b0553–b0655; double gene dosage.
Genome		StrR 2dGalRLeucineArabinose, Lactose, (?)Galactose
DH5α

str. K-12 F– endA1 glnV44 thi-1 recA1 relA1 gyrA96(NalR) deoR+ nupG purB20 φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK–mK+), λ–

Recombination-deficient. Relaxed. Endonuclease–. Clones large plasmids. Amber suppressor UAG>CAG(Gln). Slow growth in M9 from purB-20NalRThiamine, ArginineLactose
Mach1str. W ΔrecA1398 endA1 fhuA Φ80Δ(lac)M15 Δ(lac)X74 hsdR(rK–mK+)Recombination-deficient. Endonuclease–. lacZα-complementer. T1/T5/Φ80 phage resistant.
Derivative of str. W (ATCC 9637). Patent: construction/ propertie. Genome. Genome paper.		  Lactose
BW25113str. K-12 F– Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) λ– rph-1 Δ(rhaD-rhaB)568 hsdR514(rK–mK+)lacI+ Keio collection background.
Genome.		  Arabinose, Rhamnose, Lactose
DB3.1F– gyrA462(CcdBR) endA1 glnV44 Δ(sr1-recA) mcrB mrr hsdS20(rB–, mB–) ara14 galK2 lacY1 proA2 rpsL20(SmR) xyl5 leu(leuB6(Am)?) mtl1 lacI–?CcdB-resistant by gyrase mutation, not CcdA. Recombination-deficient. Endonuclease– Amber suppressor UAG>CAG(Gln).CcdBR StrR 2dGalRProline, LeucineGalactose, Lactose, Arabinose, Xylose, Mannitol
BW29655str. K-12 F– Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) λ– Δ(envZ-ompR)520(::FRT) Δ(rhaD-rhaB)568 hsdR514(rK–mK+) rph-1?lacI+  Arabinose, Lactose, Rhamnose



Shyam Can Obtain:

NameGenotype / PhenotypeFeaturesResistancesAuxotrophiesNon-utilizations
DH1str. K-12 F– λ– endA1 recA1 relA1 gyrA96(NalR) thi-1 glnV44(supE44) hsdR17(rK–mK–)Recombination-deficient. Relaxed. Endonuclease–. Efficient 40–60kb plasmid transformer. Amber suppressor UAG>CAG(Gln)
DH5α parent		NalRThiamine 
JM109str. K-12 endA1 recA1 relA1 gyrA96(NalR) thi-1 glnV44(supE44) Δ(lac-proAB) hsdR17(rK–mK+) F′ [traD36 proAB+ lacIq lacZΔM15]Recombination-deficient. Relaxed. Endonuclease–. Amber suppressor UAG>CAG(Gln)NalRThiamine 
BL21(DE3)str. B F– ompT gal dcm lon hsdSB(rB–mB–) λ(DE3 [lacI lacUV5-T7p07 ind1sam7 nin5]) [malB+]K-12(λS)proteases–; lac-inducible T7 RNAP  Galactose
BL21(DE3) pLysSstr. BL21(DE3) pLysS[T7p20 orip15A](ChlR)proteases–; lac-inducible T7 RNAP
pLysS encodes T7 lysozyme, inhibitor of T7 polymerase, which almost eliminates expression from uninduced T7 promoter.		ChlR Galactose
Rosetta™(DE3)pLysS (Novagen)BL21(DE3) pLysSRARE[T7p20 ileX argU thrU tyrU glyT thrT argW metTleuW proL orip15A](ChlR)proteases–; lac-inducible T7 RNAP
pLysSRARE Expresses rare-codon Arg, Ile, Gly, Leu and Pro-tRNAs, argU(AGG, AGA), ileX(AUA), leuW(CUA), proL(CCC), glyT(GGA), in addition to T7 lysozyme.		ChlR Galactose
BLR(DE3) (Novagen®)str. B F– ompT hsdSB(rK–mB–) gal dcm (DE3) Δ(srl-recA)306::Tn10 (TetR)protease OmpT–. Recombination-deficient.TetR Galactose
BLR(DE3) pRIL (Novagen®)str. B F– ompT hsdSB(rK–mB–) gal dcm (DE3) Δ(srl-recA)306::Tn10 (TetR) pRIL[argU ileY leuW]protease OmpT–. Recombination-deficient.
Overexpresses rare-codon tRNAArg, Ile, Leu argU (AGA, AGG), ileY (AUA), leuW (CUA)		ChlR TetR Galactose
DP10str. DH10B Δ(araFGH) φ(ΔaraEp Pcp8-araE(araEq)Deletion of Arabinose consumption genes. Constitutive araE. Others, see DH10B.StrRLeucineArabinose, Lactose
RU1012str. MH215[MH70[MC4100 [F– [araD139]B/r Δ(argF-lac)169 e14– flhD5301 Δ(fruK-yeiR)725(fruA25) relA1 rpsL150(StrR) rbsR22 Δ(fimB-fimE)632::IS1deoC1 λ–] malQ7] Φ(ompC-lacZ)10-25] ΔenvZ::KanRRelaxedStrR KanRArginineLactose, Fructose
JTK165JJ (DIAL strain)str. MC1061 Δupp:(pir dhfr(TmpR)) Δ(rfbB–wbbL)::repA

1) Δupp::(Pcon(BBa_J23100)-rbs(?)-pir-BBa_B0015(rrfBt-T7_TE) - FRT - Pcon(BBa_J23119)-4krbs-dhfr (TmpR)-tR2 - FRT)
2) Δ(rfbB–wbbL')::(Pcon(BBa_J23119)-rbs(?)-repA-BBa_B0015(rrfBt-T7_TE) - FRT)		relaxed. lacI–. Propagates R6K and ColE2 replicons. Improved cloning 5-mC DNA.StrR TmpRLeucineGalactose, Lactose, Arabinose
DGF-298
3.62 Mbp source strain		str. K-12 W3110, Δ(recC ptr recB recD)::Plac-bet exo kan ilvG+ rph+ Δ(hella shit)2.98 Mbp reduced genome (from 4.6 Mbp)---
BW27783str. K-12 BW25113 F– λ– Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) Δ(araH-araF)570(::FRT) ΔaraEp-532::FRT φPcp8-araE535 rph-1 Δ(rhaD-rhaB)568hsdR514Constitutive araE (ara transporter) expression, expanding dynamic range of araBp (PBAD) induction--Arabinose, Rhamnose, Lactose
RE630EcNR1[MG1655 bla bio λ-Red1] ilvG+ dam@λTerm tetO2-pTet<>{kil, λ cI}cymR<>SS7 araEq araCq lacIq-1 recJ sbcB dnaG-Q576AaTc-inducible recombination machinery. Recombination-deficient. Mutations to stabilize ssDNAAmpRBiotin 
      

 

 

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