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Mostly written by Shyam Bhakta ShyamPBhakta (άτ)gmαιl•com

Media

LB (Lysogeny Broth/Agar)

M9 Medium

MinA Medium

Terrific Broth (TB) & 2×YT

Super-Optimal Broth (SOB/SOC)

Other Reagents

Phosphate-Buffered Saline (PBS)

Common Stocks, Antibiotics

Reusables

Glass Beads

Multiwell Plates, Blocks, Reservoirs, Seals

Digital Tools

Wetlab Calculator; Cloning Log

Bioinformatics Bookmarks

PCR

Q5/Phusion PCR

Colony PCR

Mutagenesis

DNA Agarose Gel Electrophoresis

DNA Purification

Gel Purification

Column Purification

Plasmid Miniprep

DNA Ethanol Precipitation

Microvolume Spectrophotometry

DNA Assembly

Golden Gate Assembly

DNA Restriction Digest

DNA Ligation

Oligo Assembly

Polymerase Chain Assembly

Gibson Assembly, CPEC, SLIC

DNA Transformation

Heat-shock/chemical transformation (CCMB80 method)

Heat-shock/chemical transformation (TSS method)

Heat-shock/chemical transformation (Inoue method)

Old heat/chemical transformation (TSS method ±KCM)

Electrotransformation

Sucrose-wash electrotransformation

Genotyping

Colony PCR

DNA Restriction Digest

DNA Sequencing

Genome Modifications

Recombineering with λ red

Microfluidics / Microscopy

Shipley 1813

E-BEAM

SU-8 Photolithography

Soft Lithography

Scope Run Setup

Other Protocols

Good Pipetting Technique 


Cloning and wetbench protocols of DLS