Title: Q5/Phusion PCR  
Author: Shyam Bhakta Apr 25, 2017
Last Changed by: Shyam Bhakta Apr 17, 2024
Tiny Link: (useful for email) https://wiki.rice.edu/confluence/x/vyCDAQ
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Bennett Lab Wiki (2)
    Page: Colony PCR
    Page: Protocols
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Apr 17, 2024 13:32 Shyam Bhakta View Changes
Apr 16, 2024 15:48 Shyam Bhakta View Changes
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Feb 15, 2023 02:57 Shyam Bhakta  
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External Links (33)
    https://doi.org/10.1038/nprot.2008.133
    https://wiki.rice.edu/confluence/users/viewuserprofile.acti…
    https://doi.org/10.1093/nar/19.14.4008
    https://doi.org/10.2144/19962003478
    https://doi.org/10.2144/02324rr04
    https://bmcbiotechnol.biomedcentral.com/articles/10.1186/14…
    https://www.future-science.com/doi/full/10.2144/000113203
    https://doi.org/10.1093/nar/18.24.7465
    https://www.neb.com/products/m0530-phusion-high-fidelity-dn…
    https://www.minipcr.com/classroom-tips/four-degree-myth-pcr…
    https://www.agilent.com/cs/library/usermanuals/public/60038…
    https://github.com/shyambhakta/wetlabcalc
    https://doi.org/10.1371/journal.pone.0169774
    cshprotocols.cshlp.org/content/2006/1/pdb.prot4093
    bitesizebio.com/19420/just-what-do-all-these-additives-do/
    https://doi.org/10.1371/journal.pone.0011024
    https://doi.org/10.1016/j.bbrc.2006.06.151
    bitesizebio.com/2592/better-than-betaine-pcr-additives-that…
    https://academic.oup.com/clinchem/article/50/7/1256/5640008
    bitesizebio.com/24/pcr-problems-try-an-additive/
    https://www.neb.com/products/m0491-q5-high-fidelity-dna-pol…
    https://doi.org/10.7171/jbt.12-2301-004
    https://pcrfidelityestimator.neb.com/#!/
    https://doi.org/10.1186/1756-0500-5-257
    https://www.neb.com/tools-and-resources/usage-guidelines/ac…
    https://www.neb.com/protocols/2013/12/13/pcr-using-q5-high-…
    tmcalculator.neb.com/#!/main
    https://doi.org/10.1093/nar/20.17.4675
    https://www.cellagentech.com/content/product_files/Vazyme/P…
    https://doi.org/10.2144/000113552
    https://doi.org/10.1080/15216549800201172
    tmcalculator.neb.com/
    https://doi.org/10.2144/97224bm14